control strain atcc 700603  (ATCC)

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Assessment of PAS effects on planktonic K. quasipneumoniae ATCC 700603. A – E Bacterial growth curves under different treatment conditions (as indicated in the diagrams). The concentrations of CAZ as the fold change in the MIC (MIC CAZ = 32 mg/L) and phage titers are indicated in the legends. The data are presented as the means ± standard deviations (SDs, n = 3)

Article Snippet: Notably, the widely used quality-control strain ATCC 700603, originally labeled K. pneumoniae , has since been reclassified as K. quasipneumoniae subsp. similipneumoniae (hereafter referred to simply as K. quasipneumoniae ) [ ].

Techniques:

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Quantitative evaluation of treatment effects on K. quasipneumoniae ATCC 700603 biofilms following exposure to CAZ (0.25 × MIC), phage vB_KpUKJ_2 (10 8 PFU/mL), or their combination. A Surface area covered (SAC) by viable biofilm (in %) over time, as determined by the calcein fluorescence signal after staining with CAM. A total pixel area of 2048 × 2048 µm was analyzed from LSFM micrographs; B AUC representation of the kinetic data from ( A ) over a treatment period of 0–24 h, based on relative fluorescence units (RFU) × h; C Viable bacterial counts (CFU/mL) after biofilm treatments at three distinct time points. Statistical analysis was performed via two-way ANOVA followed by Tukey’s multiple comparisons test. D Phage particles (PFU/mL) after combined biofilm treatment at three time points. Statistical analysis was conducted via one-way ANOVA followed by Dunnett’s post hoc test. The data are presented as the means ± SDs (n = 3). Significance was assumed at p < 0.05 and is indicated by asterisks: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001

Article Snippet: Notably, the widely used quality-control strain ATCC 700603, originally labeled K. pneumoniae , has since been reclassified as K. quasipneumoniae subsp. similipneumoniae (hereafter referred to simply as K. quasipneumoniae ) [ ].

Techniques: Fluorescence, Staining

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Representative CLSM images showing the effects of phage vB_KpUKJ_2 (10 8 PFU/mL) on mature K. quasipneumoniae ATCC 700603 biofilms at 0, 4, and 24 h post-treatment. Biofilms were stained with Con-A (green) for α-polysaccharides or with CFW (magenta) for β-polysaccharides to visualize extracellular matrix integrity. A Untreated control, B phage-treated samples. To minimize photobleaching and laser-induced biofilm disruption, independent samples were used for each time point. Each image represents a representative Z-stack projection from three independent experiments. Scale bars: 20 μm

Article Snippet: Notably, the widely used quality-control strain ATCC 700603, originally labeled K. pneumoniae , has since been reclassified as K. quasipneumoniae subsp. similipneumoniae (hereafter referred to simply as K. quasipneumoniae ) [ ].

Techniques: Staining, Control, Disruption

Journal: Journal of Biomedical Science

Article Title: Phage–antibiotic synergy restores β-lactam efficacy in MDR Klebsiella quasipneumoniae biofilms and suppresses resistance

doi: 10.1186/s12929-026-01218-1

Figure Lengend Snippet: Characteristics of six phage-resistant mutants in comparison with the parental K. quasipneumoniae ATCC 700603 strain. A Differences in the AUCs based on growth kinetics. B Biofilm formation ability at 48 h quantified by crystal violet staining and absorbance measured at 570 nm; the data are presented as the means ± SDs (n = 3). Statistical analyses were performed via one-way ANOVA followed by Tukey’s multiple comparisons test. Significance was assumed at p < 0.05 and is indicated by asterisks: * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0. 0001

Article Snippet: Notably, the widely used quality-control strain ATCC 700603, originally labeled K. pneumoniae , has since been reclassified as K. quasipneumoniae subsp. similipneumoniae (hereafter referred to simply as K. quasipneumoniae ) [ ].

Techniques: Comparison, Staining